IP Result of anti-alpha Tubulin (IP: 66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. SA00001-2 (HRP-conjugated Goat Anti-Rabbit IgG(H+L) ) as the secondary antibody.
Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. SA00001-1 (HRP-conjugated Goat Anti-Mouse IgG(H+L) as secondary antibody.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10427-2-AP (Calnexin antibody) at dilution of 1:50 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Beta Actin (20536-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.
Immunofluorescent analysis of HepG2 cells using 19842-1-AP (hIST1 antibody) at dilution of 1:25 and SA00013-4 CoraLite594-conjugated Goat Anti-Rabbit IgG(H+L).
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human MYH9 (11128-1-AP) and Fluorescein (FITC)–conjugated Goat Anti-Rabbit IgG(H+L) (SA00003-2) at dilution 1:200 (red), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.
Immunofluorescent analysis of (-20 ℃ Ethanol) fixed HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65064-1-Ig, Clone: F10-89-4 ) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) at dilution 1:1000 (green), or 0.5 ug Mouse IgG2a Isotype Control (black). Cells were not fixed.
1X10^6 HepG2 cells were stained with 0.2 ug Anti-Human MYH9 (11128-1-AP) and Cy3–conjugated Goat Anti-Rabbit IgG(H+L) (SA00009-2) at dilution 1:100 (red) Control Antibody in black. Cells were fixed with 4% PFA with 0.1 trition.
1X10^6 HeLa cells were stained with 0.2 ug Lamin B1 antibody (66095-1-Ig, red) and Fluorescein (FITC)–conjugated Goat Anti-Mouse IgG(H+L) (SA00003-1) with dilution 1:100. Control antibody (blue). Cell were fixed with 90% MeOH.
1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and Cy3–conjugated Goat Anti-Mouse IgG(H+L) (SA00009-1) at dilution 1:50 (green), and 0.5 ug Control Antibody. Cells were not fixed.
Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and SA00007-2 (Rhodamine (TRITC)–conjugated Goat Anti-Rabbit IgG(H+L) (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IgG proteins of various species were subjected to SDS PAGE followed by western blot with SA00001-4 (HRP-conjugated Rabbit Anti-Goat IgG(H+L) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60293 (OPTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-0 (HRP-conjugated Streptavidin) as secondary antibody, at dilution of 1:3000 (lane 1 and 3) or 1:6000 (lane 2 and 4).
Immunofluorescent analysis of MCF-7 cells, using B23 antibody 60096-1-lg at 1:25 dilution and SA00007-1 (Rhodamine (TRITC)–conjugated Goat Anti-Mouse IgG(H+L) (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IgG proteins of various species were subjected to SDS PAGE followed by western blot with SA00001-3 (HRP-conjugated Donkey Anti-Goat IgG(H+L) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b (65052-1-Ig, Clone:ABL-93) and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) (SA00003-11) at dilution 1:50 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH.
