HumanKine® recombinant human TPO (Thrombopoietin) protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

100-500 ng/mL EC50 in MO7e cells in defined media

Specific Activity

>1.00 x 10⁶ IU/mg

Species Reactivity

human

Purity

>95%

Publications

8

Cat no : HZ-1248

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

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Validation Data Gallery

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  Purity of recombinant human TPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  The activity was determined by the dose- dependent stimulation of the proliferation of MO7e cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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  Recombinant human TPO (HZ-1248) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of GMP recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL​.

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  Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

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Technical Specifications

• GeneID: 7066
• Species: Human
• Expression: HEK293
• Activity(Primary): 100-500 ng/mL EC50 in MO7e cells in defined media
• Activity(Secondary): 1-7 ng/mL EC50 in MO7e cells
• Specific Activity: >1.00 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: < 1 EU/µg
• Accession Number: P40225
• Molecular Mass: 70 to 93 kDa reduced and non-reduced, monomer, glycosylated.
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

TPO (Thrombopoietin) is a primary regulator of megakaryocyte development and platelet production in mammals. Human Thrombopoietin constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). Thrombopoietin also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Publications

Species	Title
human	Blood; PF4 activates the c-Mpl-Jak2 pathway in platelets; Authors - Richard John Buka
human	J Exp Med; Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.; Authors - AndrewVolk
human	J Cell Physiol; HCMV modulates c-Mpl/IEX-1 pathway-mediated megakaryo/thrombopoiesis via PDGFRα and αvβ3 receptors after allo-HSCT.; Authors - Fei-Er Feng
human	ACS Biomater Sci Eng; Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.; Authors - Lidan Chen
human	Oncotarget; Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.; Authors - JingLi
human	Sci Rep; A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.; Authors - Dezhi Zhou