HumanKine® recombinant human OSM protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.1-1.5 ng/mL

Specific Activity

minimally 3.00 x 10⁷ IU/mg

Species Reactivity

human

Purity

>95%

Publications

6

Reviews (3)

Cat no : HZ-1030

Synonyms

oncostatin M, OSM

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Validation Data Gallery

• 

  Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human OSM (HZ-1030) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.5 ng/mL​.

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Technical Specifications

• GeneID: 5008
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.1-1.5 ng/mL
• Specific Activity: minimally 3.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P13725
• Molecular Mass: 32 kDa reduced, 28 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Oncostatin M (OSM) is a member of a cytokine family that includes a leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. OSM is a growth regulator which inhibits the proliferation of some tumor cell lines. It can stimulate proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF, and GM-CSF from endothelial cells. OSM is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (PMID: 9133674; 29526757; 29363180; 21887386).

Synonyms

oncostatin M, OSM

Publications

Species	Title
human	Oncotarget; CM363, a novel naphthoquinone derivative which acts as multikinase modulator and overcomes imatinib resistance in chronic myelogenous leukemia.; Authors - BorjaGuerra
human	Invest Ophthalmol Vis Sci; Role of Oncostatin M in the Pathogenesis of Vernal Keratoconjunctivitis: Focus on the Barrier Function of the Epithelium and Interleukin-33 Production by Fibroblasts; Authors - Ishin Ninomiya
human	Cancer Biomark; Interleukin-6 and oncostatin M are elevated in liver disease in conjunction with candidate hepatocellular carcinoma biomarker GP73.; Authors - HongyanLiang
human	Int J Dermatol; Livin upregulation in keratinocytes of psoriasis patients to promote adhesion molecule expression; Authors - Delu Che
human	Skin Res Technol; Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis; Authors - Delu Che
human	Front Pharmacol; A Novel Naphthoquinone-Coumarin Hybrid That Inhibits BCR-ABL1-STAT5 Oncogenic Pathway and Reduces Survival in Imatinib-Resistant Chronic Myelogenous Leukemia Cells.; Authors - Patricia Martín-Rodríguez

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

E (Verified Customer) (06-01-2022)

I used protein for hiPSC differentiation in hepatocyte cells and it works well

• Applications: Cell culture
• Cell Tissue Type: hiPSC

FH

Toby (Verified Customer) (10-05-2021)

Recombinant Human OSM is used as part of our differentiation protocol, and is involved in the last stage in which we differentiate from a hepatoblast to a hepatocyte like cell. This product worked very well and aided with successful differentiation.

• Applications: Cell culture,Other
• Cell Tissue Type: Stem cell differentiation

FH

Dongling (Verified Customer) (11-12-2018)

• Applications: Flow Cytometry,Cell culture,Other,
• Cell Tissue Type: iPSC's