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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human OSM protein- GMP grade

EC50

0.1-1.5 ng/mL

Specific Activity

minimally 3.00 x 107 IU/mg, typically 3.07 x 107 IU/mg

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1030-GMP

Synonyms

oncostatin M, OSM

Validation Data Gallery

  • Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human OSM (HZ-1030-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.1-1.5 ng/mL​.

    GMP Recombinant human OSM (HZ-1030-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.1-1.5 ng/mL​.

Technical Specifications

GeneID 5008
Species Human
Expression HEK293
EC50 0.1-1.5 ng/mL
Specific Activityminimally 3.00 x 107 IU/mg, typically 3.07 x 107 IU/mg
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP13725
Molecular Mass 32 kDa reduced, 28 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Oncostatin M (OSM) is a member of a cytokine family that includes a leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. OSM is a growth regulator which inhibits the proliferation of some tumor cell lines. It can stimulate proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF, and GM-CSF from endothelial cells. OSM is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (PMID: 9133674; 29526757; 29363180; 21887386).

Synonyms

oncostatin M, OSM