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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 protein- GMP grade

EC50

0.8-4 ng/mL

Specific Activity

minimally 3.00 x 106 IU/mg, typically 5.96 x 106 IU/mg

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1256-GMP

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Validation Data Gallery

  • Purity of recombinant human IL-12 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-12 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human IL-12 (HZ-1256-GMP) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The HEK293 reporter cell line was treated with increasing concentrations of GMP recombinant IL-12 for 24 hours before addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.8-4.0 ng/mL.

    GMP Recombinant human IL-12 (HZ-1256-GMP) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The HEK293 reporter cell line was treated with increasing concentrations of GMP recombinant IL-12 for 24 hours before addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.8-4.0 ng/mL.

Technical Specifications

GeneID 3592 and 3593
Species Human
Expression HEK293
EC50 0.8-4 ng/mL
Specific Activityminimally 3.00 x 106 IU/mg, typically 5.96 x 106 IU/mg
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP29459 (p35 subunit) P29460 (p40 subunit)
Molecular Mass 34 and 42 kDa reduced, 57 kDa non-reduced, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35