• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 protein- GMP grade

EC50

1-5 ng/mL EC50

Specific Activity

4.7 x 10^6 IU/mg calibrated against IL-12 reference standard (NIBSC cat no: 95/544). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1256-GMP

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35



Technical Specifications

GeneID 3592
Species Human
Expression HEK293
EC50 1-5 ng/mL EC50
Specific Activity4.7 x 10^6 IU/mg calibrated against IL-12 reference standard (NIBSC cat no: 95/544). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP29459 (p35 subunit) P29460 (p40 subunit)
Molecular Mass 57 kDa, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35