HumanKine® recombinant human IL-10 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.18-2.0 ng/mL

Specific Activity

minimally 7.00 x 10⁶ IU/mg

Species Reactivity

human,mouse,pig

Purity

>95%

Publications

6

Reviews (1)

Cat no : HZ-1145

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

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Validation Data Gallery

• 

  Purity of recombinant human IL-10 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

• 

  Recombinant human IL-10 (HZ-1145) induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

• 

  Proteintech IL-10 (HZ-1145) demonstrates equivalent induction of proliferation and EC50 to leading competitors. IL-10 Induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.18-2.0 ng/mL.

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Technical Specifications

• GeneID: 3586
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.18-2.0 ng/mL
• Specific Activity: minimally 7.00 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P22301
• Molecular Mass: 19 kDa reduced, 17 kDa non-reduced, non-disulfide bonded homodimer, glycosylated
• Formulation: 10mM Tris-HCl pH 7.4 + 150mM NaCl, See Certificate of Analysis for details
• Species Reactivity: human,mouse,pig

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Background

IL-10 is a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. IL-10 is produced by a variety of mammalian cell types including T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple roles in inflammation and immunoregulation (PMID: 19161426; 9162098).

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

Publications

Species	Title
human	Stem Cell Res Ther; hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.; Authors - Aiping Zhang
	Cancer Immunol Immunother; Clinicopathological significance of peritumoral alveolar macrophages in patients with resected early-stage lung squamous cell carcinoma; Authors - Yu Tanaka
pig	Curr Issues Mol Biol; Transcriptomic Establishment of Pig Macrophage Polarization Signatures; Authors - Jing Li
	Cancer Cell; Clearance of senescent macrophages ameliorates tumorigenesis in KRAS-driven lung cancer; Authors - Scott Haston
human	Cancer Lett; Hypoxia-induced TREM1 promotes mesenchymal-like states of glioma stem cells via alternatively activating tumor-associated macrophages; Authors - Minhai Dong
human	Gut Microbes; Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics; Authors - Li-Wei Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Farhat (Verified Customer) (03-08-2024)

We are using this cytokine to culture primary monocytes in vitro. The Humankine range of cytokines are amongst the best for cell culture of primary cells and we have always used them.

• Applications: Cell culture
• Cell Tissue Type: Monocytes