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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-10 protein- GMP grade

EC50

0.18-2.0 ng/mL

Specific Activity

minimally 7.00 x 106 IU/mg, typically 7.50 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1145-GMP

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

Validation Data Gallery

  • Purity of GMP recombinant human IL-10 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP recombinant human IL-10 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human IL-10 (HZ-1145-GMP) induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of GMP recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.18-2.0 ng/mL

    GMP Recombinant human IL-10 (HZ-1145-GMP) induces dose-dependent proliferation of the MC/9 (mouse mast cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. MC/9 cells were treated with increasing concentration of GMP recombinant IL-10 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.18-2.0 ng/mL

Technical Specifications

GeneID 3586
Species Human
Expression HEK293
EC50 0.18-2.0 ng/mL
Specific Activityminimally 7.00 x 106 IU/mg, typically 7.50 x 106 IU/mg
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP22301
Molecular Mass 17 kDa, non-disulfide bonded homodimer, glycosylated
Formulation 10mM Tris-HCl pH 7.4 + 150mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-10 is a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. IL-10 is produced by a variety of mammalian cell types including T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple roles in inflammation and immunoregulation (PMID: 19161426; 9162098).

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF