• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN beta protein


0.015-0.08 ng/mL

Specific Activity

minimally 1.00 x 109 IU/mg

Species Reactivity




Cat no : HZ-1298


Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast

Technical Specifications

GeneID 3456
Species Human
Expression HEK293
Activity(Primary) 0.015-0.08 ng/mL
Specific Activityminimally 1.00 x 109 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01574
Molecular Mass 21 to 24 kDa reduced, 20 to 23 and 38 to 42 non-reduced, glycosylated
Formulation Sodium Acetate pH 4.8 + 150mM NaCl + CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile water. Gently swirl or tap vial to mix.


IFN beta is a member of type I family of interferons. It binds to the IFNα/β receptor (IFNAR), activating the Jak/ STAT pathway. Activation of this signaling pathway results in upregulating genes that inhibit viral infection and regulate MHC class I antigens. It is primarily produced by fibroblasts and monocytes. In addition to inhibiting viral infection, IFN beta is also involved in regulating and activating immune response against bacteria, parasite and tumor cells. Multiple sclerosis is characterized by a deficiency of IFN beta 1. An injectable form of IFN beta 1 is used for MS treatment (PMID: 22222875; 29258190; 8602746; 8469318).


Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast



J Innate Immun

Positive Feedback Loop of Long Noncoding RNA OASL-IT1 and Innate Immune Response Restricts the Replication of Zika Virus in Epithelial A549 Cells.

Authors - Yi Wang


Linear ubiquitination-induced necrotic tumor remodeling elicits immune evasion

Authors - Katsuhiro Sasaki

Cell Rep

Critical role of Syk-dependent STAT1 activation in innate antiviral immunity.

Authors - Shasha Liu

Microbes Infect

MicroRNA-155 triggers a cellular antiviral immune response against Chandipura virus in human microglial cells

Authors - Neha Pandey

Arch Oral Biol

Ruxolitinib altered IFN-β induced necroptosis of human dental pulp stem cells during osteoblast differentiation

Authors - Atsuko Tanaka

Cell Rep

STAT3 regulates antiviral immunity by suppressing excessive interferon signaling

Authors - Shasha Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Janina (Verified Customer) (03-28-2022)

I asked the students who worked with it and they think it works fine. No bad surprises but also nothing special happen.

  • Applications: Cell culture

Christine (Verified Customer) (12-14-2021)

I induced the expression of interferon responsive genes by treating HEK293T cells for 24 or 48h with various concentrations of IFN beta. Samples were then analysed by western blot for the detection of an IFN induced protein, which was absent at t=0, clearly induced at 24h and even better expressed at 48h. The lowest amount of IFN analysed here was 3 ng/ml. I also tried IFN alpha 2a (cat number HZ-1066) at the same time and the results were also very good, identical.

  • Applications: Cell culture
  • Cell Tissue Type: HEK293T