HumanKine® recombinant human IFN beta protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.015-0.08 ng/mL

Specific Activity

minimally 1.00 x 10⁹ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

10

Reviews (2)

Cat no : HZ-1298

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast

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Validation Data Gallery

• 

  Purity of recombinant human IFN beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human IFN beta (HZ-1298) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL​.

• 

  Proteintech IFN beta (HZ-1298) demonstrates equivalent induction of proliferation and EC50 to leading competitors. IFN beta dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL​.

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Technical Specifications

• GeneID: 3456
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.015-0.08 ng/mL
• Specific Activity: minimally 1.00 x 10^9 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P01574
• Molecular Mass: 21 to 24 kDa reduced, 20 to 23 and 38 to 42 non-reduced, glycosylated
• Formulation: Sodium Acetate pH 4.8 + 150mM NaCl + CHAPS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile water. Gently swirl or tap vial to mix.

Background

IFN beta is a member of type I family of interferons. It binds to the IFNα/β receptor (IFNAR), activating the Jak/ STAT pathway. Activation of this signaling pathway results in upregulating genes that inhibit viral infection and regulate MHC class I antigens. It is primarily produced by fibroblasts and monocytes. In addition to inhibiting viral infection, IFN beta is also involved in regulating and activating immune response against bacteria, parasite and tumor cells. Multiple sclerosis is characterized by a deficiency of IFN beta 1. An injectable form of IFN beta 1 is used for MS treatment (PMID: 22222875; 29258190; 8602746; 8469318).

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast

Publications

Species	Title
human	J Innate Immun; Positive Feedback Loop of Long Noncoding RNA OASL-IT1 and Innate Immune Response Restricts the Replication of Zika Virus in Epithelial A549 Cells.; Authors - Yi Wang
mouse	FEBS Lett; Linear ubiquitination-induced necrotic tumor remodeling elicits immune evasion; Authors - Katsuhiro Sasaki
human	Cell Rep; Critical role of Syk-dependent STAT1 activation in innate antiviral immunity.; Authors - Shasha Liu
human	Microbes Infect; MicroRNA-155 triggers a cellular antiviral immune response against Chandipura virus in human microglial cells; Authors - Neha Pandey
human	Arch Oral Biol; Ruxolitinib altered IFN-β induced necroptosis of human dental pulp stem cells during osteoblast differentiation; Authors - Atsuko Tanaka
	Cell Rep; STAT3 regulates antiviral immunity by suppressing excessive interferon signaling; Authors - Shasha Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Janina (Verified Customer) (03-28-2022)

I asked the students who worked with it and they think it works fine. No bad surprises but also nothing special happen.

• Applications: Cell culture

FH

Christine (Verified Customer) (12-14-2021)

I induced the expression of interferon responsive genes by treating HEK293T cells for 24 or 48h with various concentrations of IFN beta. Samples were then analysed by western blot for the detection of an IFN induced protein, which was absent at t=0, clearly induced at 24h and even better expressed at 48h. The lowest amount of IFN analysed here was 3 ng/ml. I also tried IFN alpha 2a (cat number HZ-1066) at the same time and the results were also very good, identical.

• Applications: Cell culture
• Cell Tissue Type: HEK293T