• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN beta protein- GMP grade

EC50

0.015-0.08 ng/mL EC50

Specific Activity

1.56 X 10^9 IU/mg calibrated against IFN beta reference standard (NIBSC cat no: 00/572). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1298-GMP

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast



Technical Specifications

GeneID 3456
Species Human
Expression HEK293
EC50 0.015-0.08 ng/mL EC50
Specific Activity1.56 X 10^9 IU/mg calibrated against IFN beta reference standard (NIBSC cat no: 00/572). Reference use only. Specific activity is not routinely tested
Purity > 95%
Endotoxin<0.1 EU/μg
Accession NumberP01574
Molecular Mass 18 to 22 kDa, glycosylated
Formulation Sodium Acetate pH 4.8 + 150mM NaCl + CHAPS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IFN beta is a member of type I family of interferons. It binds to the IFNα/β receptor (IFNAR), activating the Jak/ STAT pathway. Activation of this signaling pathway results in upregulating genes that inhibit viral infection and regulate MHC class I antigens. It is primarily produced by fibroblasts and monocytes. In addition to inhibiting viral infection, IFN beta is also involved in regulating and activating immune response against bacteria, parasite and tumor cells. Multiple sclerosis is characterized by a deficiency of IFN beta 1. An injectable form of IFN beta 1 is used for MS treatment (PMID: 22222875; 29258190; 8602746; 8469318).

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast