HumanKine® recombinant human IFN alpha 2B protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

Typically ≤ 0.12 ng/mL

Specific Activity

minimally 5.00 x 10⁷ IU/mg

Species Reactivity

human

Purity

>95%

Publications

4

Cat no : HZ-1072

Synonyms

IFN alpha 2, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

---

Validation Data Gallery

• 

  Purity of recombinant human IFN alpha 2B was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human IFN alpha 2B (HZ-1072) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN alpha 2B for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.004-0.020 ng/mL​.

---

Technical Specifications

• GeneID: 3440
• Species: Human
• Expression: HEK293
• Activity(Primary): Typically ≤ 0.12 ng/mL
• Specific Activity: minimally 5.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P01563
• Molecular Mass: 18 to 22 kDa reduced, 16 to 18 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interferon alpha 2 is a type 1 interferon secreted by virus-infected cells to protect surrounding cells from the pathogen. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761)

Synonyms

IFN alpha 2, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

Publications

Species	Title
human	Cancer Sci; Combination of tumor necrosis factor-α and epidermal growth factor induces the adrenergic-to-mesenchymal transdifferentiation in SH-SY5Y neuroblastoma cells.; Authors - Yue Huang
	Antiviral Res; Antiviral candidates against the hepatitis E virus (HEV) and their combinations inhibit HEV growth in in vitro.; Authors - Takashi Nishiyama
human	J Virol Methods; Screening of novel drugs for inhibiting hepatitis E virus replication.; Authors - Takashi Nishiyama
human	EMBO Mol Med; SLE serum induces altered goblet cell differentiation and leakiness in human intestinal organoids; Authors - Inga Viktoria Hensel