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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN alpha 2B protein- GMP grade

EC50

0.004-0.02 ng/mL

Specific Activity

minimally 5.00 x 107 IU/mg, typically 5.76 x 107 IU/mg

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1072-GMP

Synonyms

IFN alpha 2, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

Validation Data Gallery

  • Purity of recombinant human IFN alpha 2B was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IFN alpha 2B was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human IFN alpha 2B (HZ-1072-GMP) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant IFN alpha 2B for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.004-0.020 ng/mL​.

    GMP Recombinant human IFN alpha 2B (HZ-1072-GMP) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant IFN alpha 2B for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.004-0.020 ng/mL​.

Technical Specifications

GeneID 3440
Species Human
Expression HEK293
EC50 0.004-0.02 ng/mL
Specific Activityminimally 5.00 x 107 IU/mg, typically 5.76 x 107 IU/mg
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP01563
Molecular Mass 18 to 22 kDa reduced, 16 to 18 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interferon alpha 2 is a type 1 interferon secreted by virus-infected cells to protect surrounding cells from the pathogen. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761)

Synonyms

IFN alpha 2, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A