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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Transferrin protein- GMP grade

EC50

65-330 ng/mL

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1317-GMP

Synonyms

HEL-S-71p, PRO1557, PRO2086, TFQTL1

Validation Data Gallery

  • Purity of GMP recombinant human Transferrin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP recombinant human Transferrin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant GMP human Transferrin (Cat no: HZ-1317-GMP) stimulates dose-dependent proliferation of the OKT4 mouse hybridoma cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. OKT4 cells were treated with increasing concentrations of recombinant Transferrin for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 75-400 ng/mL.

    Recombinant GMP human Transferrin (Cat no: HZ-1317-GMP) stimulates dose-dependent proliferation of the OKT4 mouse hybridoma cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. OKT4 cells were treated with increasing concentrations of recombinant Transferrin for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 75-400 ng/mL.

Technical Specifications

GeneID 7018
Species Human
Expression HEK293
EC50 65-330 ng/mL
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP02787
Molecular Mass 80 kDa reduced, 70 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

N/A

Synonyms

HEL-S-71p, PRO1557, PRO2086, TFQTL1