HumanKine® recombinant human Transferrin protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

65-330 ng/mL

Species Reactivity

human

Purity

>95%

Cat no : HZ-1317

Synonyms

HEL-S-71p, PRO1557, PRO2086, TFQTL1

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Validation Data Gallery

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  Purity of recombinant human Transferrin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human Transferrin (Cat no: HZ-1317) stimulates dose-dependent proliferation of the OKT4 mouse hybridoma cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. OKT4 cells were treated with increasing concentrations of recombinant Transferrin for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 75-400 ng/mL.

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Technical Specifications

• GeneID: 7018
• Species: Human
• Expression: HEK293
• Activity(Primary): 65-330 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P02787
• Molecular Mass: 80 kDa reduced, 70 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Synonyms

HEL-S-71p, PRO1557, PRO2086, TFQTL1