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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-9 protein- GMP grade

EC50

0.1-0.6 ng/mL

Specific Activity

minimally 2.00 x 106 IU/mg, typically 2.03 x 106 IU/mg

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1240-GMP

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40

Validation Data Gallery

  • Purity of recombinant human IL-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human IL-9 (HZ-1240-GMP) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant IL-9 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.15-0.85 ng/mL​.

    GMP Recombinant human IL-9 (HZ-1240-GMP) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant IL-9 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.15-0.85 ng/mL​.

Technical Specifications

GeneID 3578
Species Human
Expression HEK293
EC50 0.1-0.6 ng/mL
Specific Activityminimally 2.00 x 106 IU/mg, typically 2.03 x 106 IU/mg
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP15248
Molecular Mass 38 to 48 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin 9 (IL-9) is a γc-family cytokine produced by T-helper 9 (Th9) cells. IL-9 promotes the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the its cognate receptor (IL9R), which activates different signal transducer and activator proteins to execute various biological processes (PMID: 29703631; 29437190; 29392411; 29391143)

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40