HumanKine® recombinant human IL-9 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.1-0.6 ng/mL

Specific Activity

minimally 2.00 x 10⁶ IU/mg

Species Reactivity

human

Purity

>95%

Publications

2

Cat no : HZ-1240

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40

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Validation Data Gallery

• 

  Purity of recombinant human IL-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human IL-9 (HZ-1240) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of recombinant IL-9 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.15-0.85 ng/mL​.

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Technical Specifications

• GeneID: 3578
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.1-0.6 ng/mL
• Specific Activity: minimally 2.00 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P15248
• Molecular Mass: 38 to 45 kDa reduced, 32 to 40 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interleukin 9 (IL-9) is a γc-family cytokine produced by T-helper 9 (Th9) cells. IL-9 promotes the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the its cognate receptor (IL9R), which activates different signal transducer and activator proteins to execute various biological processes (PMID: 29703631; 29437190; 29392411; 29391143)

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40

Publications

Species	Title
human	Nature; MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment.; Authors - Vincenzo Calvanese
human	J Toxicol Sci; Human induced pluripotent stem cell-derived mast cells useful for in vitro mast cell activation assay exhibiting phenotypes and morphological characteristics of human mast cells.; Authors - Tatsuya Ikuno