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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-1 alpha protein- GMP grade

EC50

0.125-1.25 ng/mL in D10.G4.1 cells

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1320-GMP

Synonyms

Hematopoietin 1, Pro-interleukin-1-alpha, IL1 alpha, IL1, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, preinterleukin 1 alpha, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM

Validation Data Gallery

  • Purity of GMP-grade recombinant human IL-1 alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP-grade recombinant human IL-1 alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP-grade recombinant human IL-1 alpha (Cat no: HZ-1320-GMP) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 alpha for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.125-1.250 ng/mL.

    GMP-grade recombinant human IL-1 alpha (Cat no: HZ-1320-GMP) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 alpha for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.125-1.250 ng/mL.

  • GMP recombinant human IL-1 alpha (Cat no: HZ-1320-GMP) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 8-40 ng/mL EC50.

    GMP recombinant human IL-1 alpha (Cat no: HZ-1320-GMP) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 8-40 ng/mL EC50.

Technical Specifications

GeneID 3552
Species Human
Expression HEK293
EC50 0.125-1.25 ng/mL in D10.G4.1 cells
Activity(Secondary)8-40 ng/mL in an HEK293 reporter cell line
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP01583
Molecular Mass 22 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).

Synonyms

Hematopoietin 1, Pro-interleukin-1-alpha, IL1 alpha, IL1, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, preinterleukin 1 alpha, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM