HumanKine® recombinant human IL-1 alpha protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.125-1.25 ng/mL in D10.G4.1 Cells

Species Reactivity

human,mouse

Purity

>95%

Publications

1

Cat no : HZ-1320

Synonyms

Hematopoietin 1, Pro-interleukin-1-alpha, IL1 alpha, IL1, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, preinterleukin 1 alpha, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM

---

Validation Data Gallery

• 

  Purity of recombinant human IL-1 alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human IL-1 alpha (Cat no: HZ-1320) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 alpha for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.125-1.25 ng/mL.

• 

  Recombinant human IL-1 alpha (Cat no: HZ-1320) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 8-40 ng/mL ng/mL EC50.

---

Technical Specifications

• GeneID: 3552
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.125-1.25 ng/mL in D10.G4.1 Cells
• Activity(Secondary): 8-40 ng/mL in an HEK293 reporter cell line
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01583
• Molecular Mass: 22 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).

Synonyms

Hematopoietin 1, Pro-interleukin-1-alpha, IL1 alpha, IL1, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, preinterleukin 1 alpha, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM

Publications

Species	Title
human	Skin Res Technol; Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis; Authors - Delu Che