HumanKine® recombinant human IGF-I protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

2-14 ng/mL

Specific Activity

minimally 3.00 x 10² IU/mg

Species Reactivity

human

Purity

>95%

Publications

4

Reviews (1)

Cat no : HZ-1322

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C

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Validation Data Gallery

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  Purity of recombinant human IGF-I was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human IGF-I (Cat no: HZ-1322) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

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  Proteintech IGF-I (Cat no: HZ-1322) demonstrates equivalent fold induction and a 2-fold decrease in EC50 compared to leading competitors. Recombinant human IGF-I stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

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Technical Specifications

• GeneID: 3479
• Species: Human
• Expression: HEK293
• Activity(Primary): 2-14 ng/mL
• Specific Activity: minimally 3.00 x 10^2 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P05019
• Molecular Mass: 9-10 kDa reduced and non-reduced, monomer, non-glycosylated
• Formulation: 50mM Acetate pH 4.0
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

The Insulin like growth factor-1, also known as Somatomedin-C is a growth factor which is structurally related to insulin and is an important regulator of growth and differentiation in various tissues and cell systems. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptide. The two precursor isoforms are differentially expressed by various tissues. The proteolytic cleavage of the N- and C-terminal regions results in the mature IGF-1 protein which is identical between isoforms. IGF-1 binds to IGF-1 receptor and induces receptor autophosphorylation. This further phosphorylates Insulin receptor substrate -1 (IRS-1) and activates various downstream signaling pathways including the PI3-AKT, MAPK etc. (PMID: 17354613, 17113337, 29535161)

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C

Publications

Species	Title
mouse	Sci Adv; Varying mechanical forces drive sensory epithelium formation; Authors - Mingyu Xia
human	Front Bioeng Biotechnol; Unveiling the mechanism of photothermal therapy in acne man-agement: targeting sebaceous gland ferroptosis via umbilical cord mesenchymal stem cell membrane-encapsulated Au-Ag-PDA; Authors - Ronghui Wu
human	Adv Sci (Weinh); Integration of Functional Human Auditory Neural Circuits Based on a 3D Carbon Nanotube System; Authors - Yiyun Lou
	ACS Biomater Sci Eng; Electrospun PCL Nerve Conduit Filled with GelMA Gel for CNTF and IGF-1 Delivery in Promoting Sciatic Nerve Regeneration in Rat; Authors - Hailiang Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (07-27-2022)

Differentiation to mzOPCs boost

• Applications: Cell culture
• Cell Tissue Type: iPSCs