• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IGF-I protein- GMP grade

EC50

2-14 ng/mL EC50

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1322-GMP

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C



Technical Specifications

GeneID 3479
Species Human
Expression HEK293
EC50 2-14 ng/mL EC50
Purity > 95%
Endotoxin< 0.1 EU/ug
Accession NumberP05019
Molecular Mass 10 kDa
Formulation 1x PBS pH 7.4
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

The Insulin like growth factor-1, also known as Somatomedin-C is a growth factor which is structurally related to insulin and is an important regulator of growth and differentiation in various tissues and cell systems. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptide. The two precursor isoforms are differentially expressed by various tissues. The proteolytic cleavage of the N- and C-terminal regions results in the mature IGF-1 protein which is identical between isoforms. IGF-1 binds to IGF-1 receptor and induces receptor autophosphorylation. This further phosphorylates Insulin receptor substrate -1 (IRS-1) and activates various downstream signaling pathways including the PI3-AKT, MAPK etc. (PMID: 17354613, 17113337, 29535161)

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C