• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human EGF protein- GMP grade

EC50

0.1-0.6 ng/mL EC50

Species Reactivity

human,monkey

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1326-GMP

Synonyms

Urogastrone



Technical Specifications

GeneID 1950
Species Human
Expression HEK293
EC50 0.1-0.6 ng/mL EC50
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP01133
Molecular Mass 6 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,monkey

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

EGF (epidermal growth factor) is a 6 kDA, single chain protein that serves a mitogenic factor for numerous cell types, particularly epithelial cells and fibroblasts. It is predominantly produced and secreted by the kidneys in humans. EGF activates cells through binding with its corresponding receptor (EGFR). Receptor binding activates several downstream pathways leading to cell proliferation, differentiation, and apoptosis. EGF plays important roles in embryonic development, tissue regeneration, transport through ion channels, and wound healing. Dysregulated EGF signaling has been linked with renal disease progression, and EGF levels could potentially be used as biomarker to monitor disease-related cell damage (PMID: 15110798, 24513230, 27868030).

Synonyms

Urogastrone