pan-keratin Polyclonal antibody, PBS Only

pan-keratin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26411-1-PBS

Synonyms

CK 5, Cytokeratin 5, CK-5, keratin 5, KRT5

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Validation Data Gallery

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  WB analysis using 26411-1-AP (same clone as 26411-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26411-1-AP (pan-keratin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens).. This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens).. This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brown disease using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brown disease using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 26411-1-AP (same clone as 26411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 26411-1-AP (same clone as 26411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 26411-1-AP (same clone as 26411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 26411-1-AP (same clone as 26411-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 26411-1-AP (same clone as 26411-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26411-1-PBS in a different storage buffer formulation.

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Product Information

26411-1-PBS targets pan-keratin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: pan-keratin fusion protein Ag24184
• Full Name: keratin 5
• Calculated Molecular Weight: 590 aa, 62 kDa
• Observed Molecular Weight: 46-58 kDa
• GenBank Accession Number: BC024292
• Gene Symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Pan-keratin, also known as pan-cytokeratin, refers to a group of antibodies that recognize a broad array of keratin proteins. Keratins are a large family of intermediate filament (IF) proteins that provide structural integrity to epithelial cells. These proteins are essential for maintaining cell shape, strength, and resilience . The keratin gene family is divided into two major groups: type I keratins (acidic) and type II keratins (basic), with 27 type I and 26 type II keratin genes in humans. Pan-keratin immunostaining is widely used in pathology and research due to its ability to detect a variety of keratin proteins, making it a valuable tool for identifying epithelial cells and tissues. This staining technique is particularly useful in the diagnosis of tumors, as it can help distinguish between epithelial and non-epithelial neoplasms. This antibody is a pan-keratin antibody.