pan Ras Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

pan Ras Monoclonal Antibody for WB, IP, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IP, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

4H4G7

Cat no : 60309-1-PBS

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, pan Ras, RASK2

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Validation Data Gallery

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  WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60309-1-Ig (same clone as 60309-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 60309-1-Ig (same clone as 60309-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60309-1-Ig (same clone as 60309-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 60309-1-Ig (same clone as 60309-1-PBS)

  IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP80009-1

  Cytometric bead array standard curve of MP80009-1, KRAS Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60309-1-PBS. Detection antibody: 81615-1-PBS. Standard: Ag2700. Range: 0.391-50 ng/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60309-1-PBS targets pan Ras as part of a matched antibody pair:

MP80009-1: 60309-1-PBS capture and 81615-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, rabbit, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: pan Ras fusion protein Ag2700
• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 188 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC013572
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.