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  • KD/KO Validated

pan Ras Monoclonal antibody

pan Ras Monoclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugate

Unconjugated

CloneNo.

4H4G7

Cat no : 60309-1-Ig

Synonyms

KRAS, KRAS4A, KRAS4B, EC:3.6.5.2, c-K-ras



Tested Applications

Positive WB detected infetal human brain tissue, pig brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60309-1-Ig targets pan Ras in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen pan Ras fusion protein Ag2700
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated Molecular Weight 188 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2881422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Protocols

Product Specific Protocols
WB protocol for pan Ras antibody 60309-1-IgDownload protocol
IHC protocol for pan Ras antibody 60309-1-IgDownload protocol
IP protocol for pan Ras antibody 60309-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.

Authors - Huafeng Zhang
humanWB

Cancer Res

KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.

Authors - Peng Yuan
  • KD Validated
humanIHC

Exp Mol Med

Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer.

Authors - Won-Ji Ryu
humanWB

J Cell Mol Med

DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells.

Authors - Wei Wang
humanWB

Mol Pharm

Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer

Authors - Xiaoxia Yang
humanWB

Oncol Rep

EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.

Authors - Chuan Lin