pan Ras Monoclonal antibody

• Featured Product
• KD/KO Validated

pan Ras Monoclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugate

Unconjugated

Publications

15

CloneNo.

4H4G7

Cat no : 60309-1-Ig

Synonyms

KRAS, KRAS4A, KRAS4B, EC:3.6.5.2, c-K-ras

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Validation Data Gallery

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  WB analysis using 60309-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60309-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60309-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 60309-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 60309-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 60309-1-Ig

  IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human breast cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 60309-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human pan Ras (60309-1-Ig, Clone:4H4G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, pig brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

60309-1-Ig targets pan Ras in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit, chicken
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: pan Ras fusion protein Ag2700
• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 188 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC013572
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• RRID: AB_2881422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Publications

Species	Application	Title
mouse	WB	Mol Cell; TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.; Authors - Huafeng Zhang
human	WB	Cancer Res; KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.; Authors - Peng Yuan ; KD Validated
human	IHC	Exp Mol Med; Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer.; Authors - Won-Ji Ryu
human	WB	J Cell Mol Med; DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells.; Authors - Wei Wang
human	WB	Mol Pharm; Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer; Authors - Xiaoxia Yang
human	WB	Oncol Rep; EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.; Authors - Chuan Lin