CoraLite® Plus 647-conjugated p70(S6K) Polyclonal antibody

• Featured Product
• KD/KO Validated

p70(S6K) Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14485

Synonyms

Phospho-p70(S6K) (Thr389), RPS6KB1, p70 S6 kinase, p70 S6K, p70(S6K), p70S6K, p70-S6K 1, P70S6K1, p70 S6K 1, p70 S6K-alpha, S6K, S6K-beta-1, Serine/threonine-protein kinase 14A, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB 1, 70 kDa ribosomal protein S6 kinase 1, EC:2.7.11.1

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Validation Data Gallery

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  FC experiment of HeLa using CL647-14485

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human p70(S6K) (CL647-14485) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-14485 targets p70(S6K) in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p70(S6K) fusion protein Ag5883
• Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-75 kDa
• GenBank Accession Number: BC053365
• Gene Symbol: p70 S6K
• Gene ID (NCBI): 6198
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59 kDa produced by alternative initiation.