p38 MAPK Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

p38 MAPK Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1C2

Cat no : 66234-1-PBS

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38 MAPK, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

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Validation Data Gallery

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  WB analysis of human heart using 66234-1-Ig (same clone as 66234-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66234-1-Ig (same clone as 66234-1-PBS)

  WB result of p38 MAPK antibody (66234-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HEK-293 cells. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 66234-1-Ig (same clone as 66234-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66234-1-PBS targets p38 MAPK in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: p38 MAPK fusion protein Ag5797
• Full Name: mitogen-activated protein kinase 14
• Calculated Molecular Weight: 360 aa, 41 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC031574
• Gene Symbol: MAPK14
• Gene ID (NCBI): 1432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.