CoraLite® Plus 488-conjugated p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16815

Synonyms

BCAR1, CRK associated substrate, CASS1, CAS1, CAS

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Validation Data Gallery

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  IF Staining of HeLa using CL488-16815

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 p130Cas / BCAR1 antibody (CL488-16815) at dilution of 1:200.

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  FC experiment of A431 using CL488-16815

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human p130Cas (CL488-16815) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16815 targets p130Cas / BCAR1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p130Cas / BCAR1 fusion protein Ag10354
• Full Name: breast cancer anti-estrogen resistance 1
• Calculated Molecular Weight: 870 aa, 93 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC062556
• Gene Symbol: BCAR1
• Gene ID (NCBI): 9564
• RRID: AB_2919141
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C