p130Cas / BCAR1 Polyclonal antibody

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• p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

p130Cas / BCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16815-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

BCAR1, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1, CASS1

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  WB analysis using 16815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of A431 using 16815-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of RAW 264.7 using 16815-1-AP

  IP result of anti-p130Cas / BCAR1 (IP:16815-1-AP, 4ug; Detection:16815-1-AP 1:600) with RAW 264.7 cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 16815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 16815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 16815-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using p130Cas / BCAR1 antibody (16815-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A431 using 16815-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Polyclonal antibody (16815-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, HepG2 cells, T-47D cells
• Positive IP detected in: RAW 264.7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16815-1-AP targets p130Cas / BCAR1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10354

  Product name: Recombinant human BCAR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-420 aa of BC062556

  Sequence: MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA

• Full Name: breast cancer anti-estrogen resistance 1
• Calculated Molecular Weight: 870 aa, 93 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC062556
• Gene Symbol: BCAR1
• Gene ID (NCBI): 9564
• RRID: AB_2065460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56945
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody Neuropilin-1 (catalog no. 16815-1-AP) has 1 total citation. It appears in the journal Extracellular Vesicles & Circulating Nucleic Acids (IF 8.5), published in 2026. The cited study investigated how Neuropilin-1-enriched mesenchymal stem cell-derived exosomes alleviate vascular hyperpermeability in acute lung injury, using Western blot in rat samples. The research field encompasses extracellular vesicle biology, pulmonary medicine, and vascular biology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Extracell Vesicles Circ Nucl Acids; Neuropilin-1 enriched mesenchymal stem cell derived-exosomes alleviate vascular hyperpermeability in acute lung injury.; Authors - Qinyi Deng