p120 Catenin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

p120 Catenin Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F7H8

Cat no : 66208-1-PBS

Synonyms

CAS, Catenin delta 1, CTNND, CTNND1, KIAA0384, p120, p120 Catenin, p120(cas), p120(ctn), P120CAS, p120-catenin, P120CTN

---

Validation Data Gallery

• 

  WB analysis of fetal human brain using 66208-1-Ig (same clone as 66208-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66208-1-Ig (same clone as 66208-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66208-1-Ig (same clone as 66208-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66208-1-Ig (same clone as 66208-1-PBS)

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HeLa cells. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66208-1-Ig (same clone as 66208-1-PBS)

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HEK-293 cells. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66208-1-Ig(p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66208-1-PBS targets p120 Catenin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: p120 Catenin fusion protein Ag2824
• Full Name: catenin (cadherin-associated protein), delta 1
• Calculated Molecular Weight: 948 aa, 105 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC010501
• Gene Symbol: p120 Catenin
• Gene ID (NCBI): 1500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).