p120 Catenin Monoclonal antibody

• Featured Product
• KD/KO Validated

p120 Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

2F7H8

Cat no : 66208-1-Ig

Synonyms

CTNND1, CTNND, Catenin delta-1, Catenin delta 1, CAS

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Validation Data Gallery

• 

  WB analysis of fetal human brain using 66208-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66208-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66208-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66208-1-Ig

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HeLa cells.

• 

  WB analysis of HEK-293 using 66208-1-Ig

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HEK-293 cells.

• 

  IHC staining of human breast cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 66208-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66208-1-Ig(p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, HeLa cells, HEK-293 cells, A549 cells, mouse brain tissue, A431 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

66208-1-Ig targets p120 Catenin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: p120 Catenin fusion protein Ag2824
• Full Name: catenin (cadherin-associated protein), delta 1
• Calculated Molecular Weight: 948 aa, 105 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC010501
• Gene Symbol: p120 Catenin
• Gene ID (NCBI): 1500
• RRID: AB_2881599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

Publications

Species	Application	Title
human	WB,IF,IP	Mol Oncol; Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1.; Authors - Wei Tian
mouse	IF	Development; Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.; Authors - Dianlei Guo
human	IP	Proc Natl Acad Sci U S A; SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.; Authors - Qing Lyu
rat	IF,WB	Biomed Pharmacother; Inhibition of autophagy and RIP1/RIP3/MLKL-mediated necroptosis by edaravone attenuates blood spinal cord barrier disruption following spinal cord injury; Authors - Bo Xu
human	WB	Clin Exp Pharmacol Physiol; Colorectal cancer cells with stably expressed SIRT3 demonstrate proliferating retardation by Wnt/β-catenin cascade inactivation; Authors - Tianyu Li

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