ChromoTek mNeonGreen-Trap Agarose beads, kit

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Cat no : ntak

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag



Product Information

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

DescriptionThe mNeonGreen-Trap Agarose Kit contains mNeonGreen-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of mNeonGreen-fusion proteins and their interacting factors.

• Clean immunoprecipitation of mNeonGreen-fusion proteins

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmNeonGreen
Binding capacity30 μg of recombinant mNeonGreen per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827593
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
mNeonGreen-Trap Agarose beads20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution