ChromoTek mNeonGreen-Trap Agarose beads, kit

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Publications

3

Cat no : ntak

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag

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Validation Data Gallery

• 

  Immunoprecipitation of mNeonGreen:Coomassie and Western Blot; Western blot indicates high effectiveness of pull-down.I: Input, FT: Flow-through, B: Bound.

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Product Information

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

• Description: The mNeonGreen-Trap Agarose Kit contains mNeonGreen-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of mNeonGreen-fusion proteins and their interacting factors.

  • Clean immunoprecipitation of mNeonGreen-fusion proteins

  • Ready-to-use

  • No heavy & light antibody chains on the SDS-PAGE

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: mNeonGreen
• Binding capacity: 30 μg of recombinant mNeonGreen per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 2 nM
• Compatibility with mass spectrometry: The mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827593
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
mNeonGreen-Trap Agarose beads	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Other Formats

mNeonGreen-Trap Agarose

mNeonGreen-Trap Magnetic Agarose kit

mNeonGreen-Trap Magnetic Agarose

Publications

Application	Title
	Cell; The ribotoxic stress response drives UV-mediated cell death; Authors - Niladri K Sinha
IP	bioRxiv; Bsc2 is a novel regulator of triglyceride lipolysis that demarcates a lipid droplet subpopulation; Authors - Natalie Ortiz Speer
IP	J Cell Biol; Tld1 is a regulator of triglyceride lipolysis that demarcates a lipid droplet subpopulation; Authors - Natalie Ortiz Speer