CoraLite® Plus 488-conjugated m6A Monoclonal antibody

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  IF Staining of mouse testis using CL488-68055

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 488 m6A antibody (CL488-68055, Clone: 1D5E10 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

m6A Monoclonal Antibody for IF-P

Cat No. CL488-68055

Clone No.1D5E10

Host / Isotype

Mouse / IgG3

Reactivity

chemical compound, m6a

Applications

IF-P

Synonyms

6-N-Methyladenoside, N6-methyladenosine

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Tested Applications

• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68055 targets m6A in IF-P applications and shows reactivity with chemical compound, m6a samples.

• Tested Reactivity: chemical compound, m6a
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: m6A
• GenBank Accession Number: m6A
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.