m6A Monoclonal antibody

m6A Monoclonal Antibody for RIP, IHC, ELISA, Dot Blot

Host / Isotype

Mouse / IgG3

Reactivity

m6A, Human, Mouse, Rat and More (4)

Applications

WB, IP, IF, RIP, IHC, ELISA, Dot Blot

Conjugate

Unconjugated

CloneNo.

1D5E10

Cat no : 68055-1-Ig

Synonyms

6-N-Methyladenoside, m6A, N6-methyladenosine, RNA methylation



Tested Applications

Positive RIP detected inRNA
Positive IHC detected inmouse testis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, rat lymph node
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive ELISA detected inm6A
Positive Dot Blot detected inRNA

Recommended dilution

ApplicationDilution
RImmunoprecipitation (IP)RIP : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:2000-1:20000
DOT BLOTDOT BLOT : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68055-1-Ig targets m6A in WB, IP, IF, RIP, IHC, ELISA, Dot Blot applications and shows reactivity with m6A, Human, Mouse, Rat samples.

Tested Reactivity m6A, Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Compound
Full Name m6A
GenBank Accession Numberm6A
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68055-1-IgDotBlot.pdf

Protocols

Product Specific Protocols
IHC protocol for m6A antibody 68055-1-IgDownload protocol
IP protocol for m6A antibody 68055-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
humanRIP

Adv Sci (Weinh)

RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation

Authors - Yu Sun
RIP

Oncogene

N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition

Authors - Rui Liu
mouseIHC

Cells

Aberrant DNA and RNA Methylation Occur in Spinal Cord and Skeletal Muscle of Human SOD1 Mouse Models of ALS and in Human ALS: Targeting DNA Methylation Is Therapeutic

Authors - Lee J Martin
human

Front Genet

Transcriptome-Wide Analysis of RNA N6-Methyladenosine Modification in Adriamycin-Resistant Acute Myeloid Leukemia Cells.

Authors - Shu Fang
humanRIP

Chem Biol Drug Des

IGF2BP3 loss inhibits cell progression by upregulating has_circRNA_103820, and hsa_circRNA_103820-encoded peptide inhibits cell progression by inactivating the AKT pathway in lung cancer

Authors - Jinrong Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tatyana (Verified Customer) (12-04-2023)

Used for dot blot on total RNA, strong signal

  • Applications: Other
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / SH-SY5Y cells
m6A Antibody Other validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:68055-1-Ig)
FH

H (Verified Customer) (08-02-2022)

This antibody works great in Human cells lines

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HCT15