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IGF2BP3 Monoclonal antibody

IGF2BP3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C10B11

Cat no : 66526-1-Ig

Synonyms

hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IGF2BP3 Monoclonal antibody (66526-1-Ig)

    WB analysis using 66526-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66526-1-Ig (IGF2BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IGF2BP3 Monoclonal antibody (66526-1-Ig)

    WB analysis using 66526-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66526-1-Ig (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using IGF2BP3 Monoclonal antibody (66526-1-Ig)

    WB analysis of HSC-T6 using 66526-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66526-1-Ig (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using IGF2BP3 Monoclonal antibody (66526-1-Ig)

    WB analysis of NIH/3T3 using 66526-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66526-1-Ig (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66526-1-Ig targets IGF2BP3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGF2BP3 fusion protein Ag6349
Full Name insulin-like growth factor 2 mRNA binding protein 3
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC065269
Gene Symbol IGF2BP3
Gene ID (NCBI) 10643
RRIDAB_2881889
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma.

Protocols

Product Specific Protocols
WB protocol for IGF2BP3 antibody 66526-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article