Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-27298 targets calreticulin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26064 Product name: Recombinant human calreticulin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 248-417 aa of BC002500 Sequence: KKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL Predict reactive species |
| Full Name | calreticulin |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC002500 |
| Gene Symbol | Calreticulin |
| Gene ID (NCBI) | 811 |
| RRID | AB_2934966 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27797 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 calreticulin antibody CL647-27298 | Download protocol |
| IF protocol for CL Plus 647 calreticulin antibody CL647-27298 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The calreticulin Polyclonal antibody (CL647-27298) has 4 published citations appearing in ACS Appl Mater Interfaces, Biomaterials Advances, iScience, and Current Biology. The associated research fields include photothermal/chemodynamic therapy with antimetastatic immunity, localized chemo-immunotherapy for head and neck squamous cell carcinoma, CD47 ubiquitination and macrophage-mediated erythrocyte clearance, and ER–multivesicular body contact-dependent exosome biogenesis.
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Coordination Self-Assembly of a Carrier-Free Hyaluronic Acid-Modified Nanoplatform for Augmented Photothermal/Chemodynamic Therapy and Robust Antimetastatic Immunity. | ||
Biomater Adv Localized chemo-immunotherapy for HNSCC via CpG-synergized doxorubicin release from injectable TPGS/Na₂CO₃ hydrogel remodeling tumor immunity. | ||
iScience Ubiquitination and degradation of CD47 enhances macrophage phagocytosis of hemolytic erythrocytes. | ||
Curr Biol BLTP2 bridges the ER and MVB for exosome biogenesis at SCAMP3-dependent ER contacts. |
