cIAP2 Recombinant antibody

cIAP2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

242214E11

Cat no : 84779-4-RR

Synonyms

BIRC3, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3, Baculoviral IAP repeat-containing protein 3

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Validation Data Gallery

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  WB analysis using 84779-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84779-4-RR (cIAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 84779-4-RR

  IP result of anti-cIAP2 (IP:84779-4-RR, 4ug; Detection:84779-4-RR 1:1000) with Raji cells lysate 1600 ug.

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  FC experiment of Raji using 84779-4-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug cIAP2 Recombinant antibody (84779-4-RR, Clone:242214E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells
• Positive IP detected in: Raji cells
• Positive FC (Intra) detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84779-4-RR targets cIAP2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: cIAP2 fusion protein Ag20500
• Full Name: baculoviral IAP repeat-containing 3
• Calculated Molecular Weight: 604 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC027485
• Gene Symbol: cIAP2
• Gene ID (NCBI): 330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.