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cGAS Monoclonal antibody, PBS Only

cGAS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G2C5

Cat no : 68640-1-PBS

Synonyms

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2G2C5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cGAS Monoclonal antibody (68640-1-Ig)

    WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using cGAS Monoclonal antibody (68640-1-Ig)

    WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using cGAS Monoclonal antibody (68640-1-Ig)

    IF Staining of HeLa using 68640-1-Ig (same clone as 68640-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (68640-1-Ig, Clone: 2G2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

Product Information

68640-1-PBS targets cGAS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen cGAS fusion protein Ag30074
Full Name chromosome 6 open reading frame 150
Calculated Molecular Weight 496 aa, 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC113608
Gene Symbol cGAS
Gene ID (NCBI) 115004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.