cGAS Monoclonal antibody

cGAS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2G2C5

Cat no : 68640-1-Ig

Synonyms

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2G2C5

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Validation Data Gallery

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  WB analysis using 68640-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68640-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68640-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (68640-1-Ig, Clone: 2G2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: NK-92 cells, NCI-H1299 cells, SCaBER cells, A431 cells, THP-1 cells, Mo7e cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68640-1-Ig targets cGAS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: cGAS fusion protein Ag30074
• Full Name: chromosome 6 open reading frame 150
• Calculated Molecular Weight: 496 aa, 54 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC113608
• Gene Symbol: cGAS
• Gene ID (NCBI): 115004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Publications

Species	Application	Title
mouse	WB	Environ Pollut; Disturbance of Mitochondrial Dynamics Led to Spermatogenesis Disorder in Mice Exposed to Polystyrene Micro- and Nanoplastics; Authors - Moxuan Zhao