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cGAS Monoclonal antibody

cGAS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G2C5

Cat no : 68640-1-Ig

Synonyms

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2G2C5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using cGAS Monoclonal antibody (68640-1-Ig)

    WB analysis using 68640-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using cGAS Monoclonal antibody (68640-1-Ig)

    WB analysis using 68640-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using cGAS Monoclonal antibody (68640-1-Ig)

    IF Staining of HeLa using 68640-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (68640-1-Ig, Clone: 2G2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inNK-92 cells, NCI-H1299 cells, SCaBER cells, A431 cells, THP-1 cells, Mo7e cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68640-1-Ig targets cGAS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen cGAS fusion protein Ag30074
Full Name chromosome 6 open reading frame 150
Calculated Molecular Weight 496 aa, 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC113608
Gene Symbol cGAS
Gene ID (NCBI) 115004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Protocols

Product Specific Protocols
WB protocol for cGAS antibody 68640-1-IgDownload protocol
IF protocol for cGAS antibody 68640-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Environ Pollut

Disturbance of Mitochondrial Dynamics Led to Spermatogenesis Disorder in Mice Exposed to Polystyrene Micro- and Nanoplastics

Authors - Moxuan Zhao
View Article