c-MYC Polyclonal antibody, PBS Only

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  WB analysis of A549 using 10828-1-AP (same clone as 10828-1-PBS)

  WB result of c-MYC antibody (10828-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-MYC transfected A549 cells. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10828-1-AP (same clone as 10828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10828-1-AP (same clone as 10828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10828-1-AP (same clone as 10828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10828-1-AP (same clone as 10828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 10828-1-AP (same clone as 10828-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10828-1-AP (same clone as 10828-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 10828-1-AP (same clone as 10828-1-PBS)

  IP result of anti-c-MYC (IP:10828-1-AP, 4ug; Detection:10828-1-AP 1:1000) with MCF-7 cells lysate 1660 ug. This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 10828-1-AP (same clone as 10828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10828-1-AP (c-MYC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 10828-1-AP (same clone as 10828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using c-MYC antibody (10828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10828-1-AP (same clone as 10828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  IF Staining of Saos-2 using 10828-1-AP (same clone as 10828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Saos-2 using 10828-1-AP (same clone as 10828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 10828-1-AP (same clone as 10828-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (10828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

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  FC experiment of NCCIT using 10828-1-AP (same clone as 10828-1-PBS)

  1x10^6 NCCIT cells were intracellularly stained with 0.25 ug c-MYC Polyclonal antibody (10828-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 10828-1-AP (same clone as 10828-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Polyclonal antibody (10828-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10828-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

c-MYC Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10828-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

MYC, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL

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Product Information

10828-1-PBS targets c-MYC in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-MYC fusion protein Ag1263
• Full Name: v-myc myelocytomatosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 62-65 kDa, 50 kDa
• GenBank Accession Number: BC000141
• Gene Symbol: MYC
• Gene ID (NCBI): 4609
• RRID: AB_2148585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01106
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct. The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.