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CoraLite® Plus 750-conjugated c-Cbl Monoclonal antibody

c-Cbl Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2F6D1

Cat no : CL750-66576

Synonyms

C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55



Tested Applications

Positive WB detected inRaji cells, mouse brain tissue, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66576 targets c-Cbl in WB applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-Cbl fusion protein Ag23033
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC132733
Gene Symbol CBL
Gene ID (NCBI) 867
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 c-Cbl antibody CL750-66576Download protocol
Standard Protocols
Click here to view our Standard Protocols