c-Cbl Monoclonal antibody

• Featured Product
• KD/KO Validated

c-Cbl Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2F6D1

Reviews (1)

Cat no : 66576-1-Ig

Synonyms

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55

---

Validation Data Gallery

• 

  WB analysis using 66576-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66576-1-Ig (c-Cbl antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66576-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66576-1-Ig (CBL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66576-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66576-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: c-Cbl fusion protein Ag23033
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC132733
• Gene Symbol: CBL
• Gene ID (NCBI): 867
• RRID: AB_2881936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Publications

Species	Application	Title
human	WB	Autophagy; RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.; Authors - Weijie Dong ; KD Validated
human	WB	Cytokine; The CBL-LSD1-CXCL8 axis regulates methionine metabolism in glioma.; Authors - Jie Chang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kus (Verified Customer) (12-08-2022)

This Ab always worked perfect for our experiments in western blotting at 1:1000 or 2000 dilution in 5% BSA+TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

c-Cbl Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:66576-1-Ig)