HRP-conjugated Beta Actin Monoclonal antibody

• Featured Product
• KD/KO Validated

Beta Actin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, zebrafish, monkey

Applications

WB, IP

Conjugate

HRP

Publications

62

CloneNo.

2D4H5

Cat no : HRP-66009

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin

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Validation Data Gallery

• 

  WB analysis using HRP-66009

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-66009 (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 60 publications below
• IP: See 1 publications below

Product Information

HRP-66009 targets Beta Actin in WB, IP applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey samples.

• Tested Reactivity: human, mouse, rat, hamster, zebrafish, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: actin, beta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001101
• Gene Symbol: Beta Actin
• Gene ID (NCBI): 60
• RRID: AB_2883836
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.

Publications

Species	Application	Title
mouse	WB	Protein Cell; Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm; Authors - Kuisheng Liu
	WB	Nat Commun; Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice; Authors - Nana Yan
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human,mouse	WB	Nat Commun; SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer.; Authors - Xiaowen Wang
human	WB	Bone Res; Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.; Authors - Dong Wang
mouse	WB	Acta Pharmacol Sin; Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice; Authors - Xiao Li