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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, monkey, zebrafish, hamster, dog

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2D4H5

Cat no : CL555-66009

Synonyms

bate actin, ACTB, β actin, 2D4H5, Actin, cytoplasmic 1, N-terminally processed

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®555-conjugated Beta Actin Monoclonal anti (CL555-66009)

    IF Staining of A549 using CL555-66009

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 Beta Actin antibody (CL555-66009, Clone: 2D4H5 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Beta Actin Monoclonal anti (CL555-66009)

    FC experiment of HeLa using CL555-66009

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Beta Actin (CL555-66009, Clone:2D4H5) (red), or 0.4 ug CoraLite® Plus 555 Mouse IgG2b Isotype Control (MPC-11) (CL555-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66009 targets Beta Actin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey, zebrafish, hamster, dog samples.

Tested Reactivity human, mouse, rat, monkey, zebrafish, hamster, dog
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name actin, beta
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol Beta Actin
Gene ID (NCBI) 60
RRIDAB_2919667
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer's disease (PMID: 11246152). 


For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.



Protocols

Product Specific Protocols
IF protocol for CL555 Beta Actin antibody CL555-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols