CoraLite®594-conjugated Beta Actin Monoclonal antibody

• Featured Product
• KD/KO Validated

Beta Actin Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, canine, monkey, zebrafish, hamster

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

21

CloneNo.

2D4H5

Cat no : CL594-66009

Synonyms

ACTB, bate actin, β actin, Beta-actin, Actin, cytoplasmic 1

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Validation Data Gallery

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  WB analysis using CL594-66009

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66009 (Beta Actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using CL594-66009

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.

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  IF Staining of MDCK using CL594-66009

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66009

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated Beta Actin Monoclonal antibody (CL594-66009, Clone:2D4H5)(red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells
• Positive IF/ICC detected in: MDCK cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IF: See 1 publications below

Product Information

CL594-66009 targets Beta Actin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine, monkey, zebrafish, hamster samples.

• Tested Reactivity: human, mouse, rat, canine, monkey, zebrafish, hamster
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: actin, beta
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001101
• Gene Symbol: Beta Actin
• Gene ID (NCBI): 60
• RRID: AB_2883475
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publications

Species	Application	Title
human		Protein Cell; The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting; Authors - Yuhan Chen
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
	WB	Hypertens Res; Unilateral chemical ablation of the adrenal gland lowers blood pressure and alleviates target organ damage in spontaneously hypertensive rats; Authors - Xinquan Wang
	WB	Inflammation; Ursolic Acid Promotes Autophagy by Inhibiting Akt/mTOR and TNF-α/TNFR1 Signaling Pathways to Alleviate Pyroptosis and Necroptosis in Mycobacterium tuberculosis-Infected Macrophages; Authors - Jingjing Shen
	WB	Neurochem Res; Akt/mTOR Pathway Agonist SC79 Inhibits Autophagy and Apoptosis of Oligodendrocyte Precursor Cells Associated with Neonatal White Matter Dysplasia; Authors - Zhongni Li
mouse	WB	J Ethnopharmacol; The licorice flavonoid isoliquiritigenin attenuates Mycobacterium tuberculosis-induced inflammation through Notch1/NF-κB and MAPK signaling pathways.; Authors - Jinxia Sun

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C