ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 568 [CTK0103, CTK0104]
Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
Applications
IF, WB, FC
Conjugate
Alexa Fluor® 568
Host
Alpaca
Reactivity
Mouse IgG1
Cat no : sms1AF568-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
Description | Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
Applications | IF, WB, FC |
Host | Alpaca |
Species Reactivity | Mouse IgG1 Fc-fragment No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3 |
Conjugate | Alexa Fluor® 568 |
Physical State | Liquid |
Suggested Dilution | Immunofluorescence 1:500 Super-resolution microscopy 1:500 Western blot 1:1,000 |
Purification Method | Recombinant expression, affinity purification IMAC |
Type | Mixture of 2 monoclonal Nanobodies |
Class | Recombinant |
Affinity (KD) | CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
RRID | AB_2827579 |
Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
SDS |
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SDS Nano-Secondary alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor 568 [CTK0103, CTK0104] (PDF) |
Protocol |
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Protocol Nano-Secondary alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor 568 [CTK0103, CTK0104] (PDF) |
Application note |
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Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
One-step incubation with Nano-Secondaries (PDF) |
Brochure |
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Chromotek nanobodies brochure (PDF) |
Publications
Application | Title |
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Biosens Bioelectron 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing | |
Cell Mol Life Sci SHANK3 deficiency leads to myelin defects in the central and peripheral nervous system. | |
Vet Microbiol NDV inhibited IFN-β secretion through impeding CHCHD10-mediated mitochondrial fusion to promote viral proliferation | |
Biochim Biophys Acta Mol Basis Dis The regulatory role of the apelin/APJ axis in scarring: Identification of upstream and downstream mechanisms | |
Cell Biol Int Fat mass and obesity-associated protein alleviates Aβ(1-40) induced retinal pigment epithelial cells degeneration via PKA/CREB signaling pathway |