ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, for 2x Cys conjugation [CTK0103]

Monovalent, recombinant secondary single domain antibody to mouse IgG1: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

Applications

IF, WB, FC

Reactivity

mouse

Host

Alpaca

Conjugate

Unconjugated

Publications

1

Cat no : smsG1Cys2-1

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

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Validation Data Gallery

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Product Information

Monovalent, recombinant secondary single domain antibody to mouse IgG1: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

• Applications: IF, WB, FC
• Host: Alpaca
• Reactivity: mouse
• Specificity/Target: Fc-fragment of mouse IgG1
• Conjugate: N- and C-terminal cysteine conjugation with thiol-reactive reagents
• Molecular weight: 14.3 kDa
• Extinction coefficient (280 nm): 25,690 M^-1*cm^-1
• Type: Nano-Secondary®, secondary Nanobody (VHH)
• Format: Alpaca single domain antibody, monovalent
• Cross-reactivity: No cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG2a, IgG2b, IgG2c, and IgG3
• Immunogen: Purified mouse IgG1
• Clonality: Monoclonal Nanobody
• Clone: CTK0103
• RRID: AB_2864263
• Affinity: K_D = 0.1 nM
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP; Preservative: 0.09 % Sodium azide
• Storage Condition: Shipped on dry ice. Store at -20°C/-4°F. Avoid freeze-thaw cycles.
• Size: 500 µg

Publications

Application	Title
IF	bioRxiv; Cell differentiation controls iron assimilation in a choanoflagellate; Authors - Fredrick Leon