ZO-1 Monoclonal antibody, PBS Only

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  WB analysis using 66452-1-Ig (same clone as 66452-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66452-1-Ig (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 66452-1-Ig (same clone as 66452-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66452-1-Ig (ZO-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ZO-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66452-1-PBS

Clone No.1G4A1

Host / Isotype

Mouse / IgG1

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1

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Product Information

66452-1-PBS targets ZO-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZO-1 fusion protein Ag16454
• Full Name: tight junction protein 1 (zona occludens 1)
• Calculated Molecular Weight: 1748 aa, 195 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC111712
• Gene Symbol: ZO-1
• Gene ID (NCBI): 7082
• RRID: AB_2881821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07157
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).