Validation Data Gallery
|Positive WB detected in||HUVEC cells, HeLa cells, COLO 320 cells, A431 cells, LNCaP cells, HEK-293 cells, HepG2 cells|
|Positive IHC detected in||human pancreas cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, MDCK cells, human breast cancer tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:750-1:3000|
|Sample-dependent, check data in validation data gallery|
66452-1-Ig targets ZO-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, Canine samples.
|Tested Reactivity||human, Canine|
|Cited Reactivity||human, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ZO-1 fusion protein Ag16454|
|Full Name||tight junction protein 1 (zona occludens 1)|
|Calculated molecular weight||1748 aa, 195 kDa|
|Observed molecular weight||230 kDa|
|GenBank accession number||BC111712|
|Gene ID (NCBI)||7082|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
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