CoraLite® Plus 488-conjugated ZIP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZIP7 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19429

Synonyms

D6S115E, D6S2244E, H2 KE4, HKE4, KE4, RING5, SLC39A7, Zinc transporter SLC39A7, ZIP7

---

Validation Data Gallery

• 

  IF Staining of HEK-293 using CL488-19429

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ZIP7 antibody (CL488-19429) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19429 targets ZIP7 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZIP7 fusion protein Ag13762
• Full Name: solute carrier family 39 (zinc transporter), member 7
• Calculated Molecular Weight: 469 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa, 56 kDa
• GenBank Accession Number: BC000645
• Gene Symbol: ZIP7
• Gene ID (NCBI): 7922
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)