ZIP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZIP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 19429-1-AP

Synonyms

KE4, RING5, SLC39A7, Zinc transporter SLC39A7, ZIP 7, ZIP7

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Validation Data Gallery

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  WB analysis using 19429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19429-1-AP (ZIP7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19429-1-AP

  IP result of anti-ZIP7 (IP:19429-1-AP, 4ug; Detection:19429-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19429-1-AP (ZIP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19429-1-AP (ZIP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19429-1-AP (ZIP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19429-1-AP (ZIP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 19429-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ZIP7 antibody (19429-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Product Information

19429-1-AP targets ZIP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZIP7 fusion protein Ag13762
• Full Name: solute carrier family 39 (zinc transporter), member 7
• Calculated Molecular Weight: 469 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa, 56 kDa
• GenBank Accession Number: BC000645
• Gene Symbol: ZIP7
• Gene ID (NCBI): 7922
• RRID: AB_10643376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)

Publications

Species	Application	Title
mouse	WB	Chem Sci; X-ray fluorescence imaging reveals subcellular biometal disturbances in a childhood neurodegenerative disorder.; Authors - A Grubman
human	WB	Cell Death Differ; Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking.; Authors - Astrid Fauster
mouse	WB,IF	Acta Neuropathol Commun; Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.; Authors - Alexandra Grubman ; KD Validated
mouse	IF	J Biol Chem; PMID: 25969539; Authors - Ying Liu
mouse	WB	Metallomics; Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses?; Authors - Alexandra Grubman
human	WB	J Nutr Biochem; B cell activation and proliferation increase intracellular zinc levels.; Authors - Johanna Ollig